Abstract
Prolonged release drug delivery system of pilocarpine nitrate was made by optimizing thin layer film hydration method. Egg phosphatidylcholine and cholesterol were used to make multilamellar vesicles. Effects of charges over the vesicles were studied by incorporating dicetylphosphate and stearylamine. Various factors, which may affect the size, shape, encapsulation efficiency and release rate, were studied. Liposomes in the size range 0.2 to 1 µm were obtained by optimizing the process. Encapsulation efficiency of neutral, positive and negatively charged liposomes were found to be 32.5, 35.4 and 34.2 percent, respectively. In vitro drug release rate was studied on specially designed model. Biological response in terms of reduction in intraocular pressure was observed on rabbit eyes. Pilocarpine nitrate liposomes were lyophilized and stability studies were conducted.
Highlights
Prolonged release drug delivery system of pilocarpine nitrate was made by optimizing thin layer film hydration method
In vitro release rate studies were conducted on specially designed in vitro model and in vivo studies of liposomes were performed on albino rabbit eyes
Phosphatidylcholine, cholesterol, dicetylphosphate and stearylamine were purchased from Sigma chemicals U.S All other chemicals and reagents used were of analytical grade
Summary
Various factors, which may affect the size, shape, encapsulation efficiency and release rate, were studied. The importance of liposomes as drug delivery vehicle is becoming well established This applies to the ability of liposomes to buffer the toxicity of entrapped drugs while maintaining efficacy[1], some areas in which liposomes display therapeutic promise are as carriers for anticancer agents[2,3,4], antiparasitic[5], antibacterial[6], antifungal drugs[7], antiviral[8,9] and ocular liposomes[10,11,12]. Multilamellar vesicles (MLVs) were prepared using thin lipid film hydration method Various factors such as phosphatidylcholine and cholesterol ratio, lipid and drug ratio, incorporation of charged species and pH etc. In vitro release rate studies were conducted on specially designed in vitro model and in vivo studies of liposomes were performed on albino rabbit eyes
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