Abstract
The systematic position of 16 yeast strains isolated from Thailand, Hungary, The Netherlands, and the Republic of Poland were evaluated using morphological, physiological, and phylogenetic analyses. Based on the similarity of the D1/D2 domain of the LSU rRNA gene, the strains were assigned to two distinct species, Trichosporiella flavificans and representatives of a new yeast species. Phylogenetic analyses revealed that Candida ghanaensis CBS 8798T showed a strong relationship with the aforementioned two species. The more fascinating issue is that Candida and Trichosporiella genera have been placed in different subphyla, Saccharomycotina and Pezizomycotina, respectively. The close relationship between Trichosporiella flavificans, Candida ghanaensis and the undescribed species was unexpected and needed to be clarified. As for morphological and physiological characteristics, the three yeast species shared a hairy colony appearance and an ability to assimilate 18 carbon sources. Based on phylogenetic analyses carried out in the present study, Crinitomyces gen. nov. was proposed to accommodate the new yeast species, Crinitomyces reliqui sp. nov. (Holotype: TBRC 15054, Isotypes: DMKU-FW23-23 and PYCC 9001). In addition, the two species Trichosporiella flavificans and Candida ghanaensis were reassigned to the genus Crinitomyces as, Crinitomyces flavificans (Type: CBS 760.79) comb. nov. and Crinitomyces ghanaensis (Type: CBS 8798) comb. nov., respectively.
Highlights
Several species concepts have been applied for yeast identification
Hundred milliliter aliquots of the samples were enriched in 500 mL yeast nitrogen base (YNB) medium supplemented with 0.5% (v/v) carbon-source and incubated on a horizontal shaker for seven days (25 ◦C, 100 rpm), 0.1 mL of each culture was transferred to a 16 mm culture tube containing 5 mL of liquid medium with the same composition
The result showed that the top two results from a BLASTn search hit with the currently recognized species Trichosporiella flavificans CBS 760.79T and Candida ghanaensis CBS 8798T, respectively. These two species are described in different subphyla i.e., T. flavificans was placed in the subphylum
Summary
Several species concepts have been applied for yeast identification. A phenotypic species concept and growth profiles were initially used, while a biological species concept including data from mating experiments was later employed [1]. A number of yeasts have been identified by phenotypic approach, often leading to misidentification or incorrect taxonomic assignment. One of these species-rich and polyphyletic yeast genera within Saccharomycetales order is the genus Candida. Phylogenetic analysis led to the recognition that various Candida species are distributed throughout the subphylum Saccharomycotina [7] This situation is aggravated by the observation that even some novel species are described in the Candida genus as a temporary placement based on short sequences and/or a small number of genes in phylogenetic analyses and, in addition, the lack of taxonomic characters needed to classify them elsewhere [8]. Many Candida species are awaiting more analysis and accurate classification
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
