Abstract
Aptamers against deoxynivalenol (DON) were selected through capture-systematic evolution of ligands by exponential enrichment. Through isothermal titration calorimetry and fluorimetric assay, aptamer candidate DN-2 demonstrated good affinity to DON with Kd value of 40.36 ± 6.32nM. Accordingly, a Forster resonance energy transfer aptasensor was fabricated by using the aptamer DN-2 combined with AuCu bimetallic nanoclusters as energy donor and MoS2 nanosheets as energy acceptor. Under the optimal conditions, the fluorescence response was utilized for DON quantitative determination ranging from 5 to 100ng/mL with a detection limit of 1.87ng/mL. The practical application of this method was verified in maize flour samples and demonstrated a satisfied recovery of 94.6 ~ 103.1%. The obtained aptamers and their application in DON determination provide a new tool for DON monitoring in various foodstuff.
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