Denitrifying anaerobic methane oxidation in marsh sediments of Chongming eastern intertidal flat.

Abstract

Denitrifying anaerobic methane oxidation (DAMO) and associated microbial diversity and abundance in the marsh sediments of Chongming eastern intertidal flat, the Yangtze Estuary, were investigated using carbon-isotope tracing and molecular techniques. Co-existence of nitrate-DAMO archaea and nitrite-DAMO bacteria was evidenced, with higher biodiversity of DAMO archaea than DAMO bacteria. Abundance of DAMO archaeal mcrA gene and DAMO bacterial pmoA gene ranged from 4.2 × 103 to 3.9 × 1010 copies g-1 and from 4.5 × 105 to 6.4 × 106 copies g-1, respectively. High DAMO potential was detected, ranging from 0.6 to 46.7 nmol 13CO2 g-1 day-1 for nitrate-DAMO and from 1.3 to 39.9 nmol 13CO2 g-1 day-1 for nitrite-DAMO. In addition to playing an important role as a CH4 sink, DAMO bacteria also removed a substantial amount of reactive nitrogen (29.4 nmol N g-1 day-1) from the intertidal sediments. Overall, these results indicate the importance of DAMO bioprocess as methane and nitrate sinks in intertidal marshes.