Abstract

ABSTRACTThe distribution of DNP groups on the surface of guinea pig lymph node cells incubated with 2.5 mM DNBSO3Na in tubes was investigated by an indirect method using scanning immunoelectron microscopy with bacteriophage T4 labelled with antibody as a visual marker. Fairly precise localization could be achieved with scanning electron microscopy. It became apparent that DNP groups were distributed in varying amounts on the surface of lymph node cells incubated with DNBSO3Na in tubes and that DNP groups had a tendency to be distributed patchily on cells sufficiently exposed. The frequency of the cells to which DNP groups attached was approximately 50%. The significance of these findings is discussed.

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