Demographic and genetic structure of a severely fragmented population of the endangered hog deer (Axis porcinus) in the Indo-Burma biodiversity hotspot.

The hog deer (Axis porcinus) is threatened by habitat alteration, fragmentation, and poaching, which have led to a drastic decline of its wild population. Two subspecies of A. porcinus have been described from its distribution range. A. p. porcinus is reported to occur from Pakistan along the Himalayan foothills through Nepal, India and Myanmar, and A. p. annamiticus is found in Thailand, Indo-China, Laos, Cambodia, and Vietnam. However, the current distribution range of A. p. annamiticus is still unclear. We used the partial control region (CR) of mitochondrial DNA (mtDNA) and seven microsatellite loci to investigate the intra-species structure, differentiation, and demographic history of hog deer populations from three landscapes, the Terai Arc, Northeast, and Indo-Burma (Keibul Lamjao National Park (KLNP), Manipur, India) landscapes. We also carried out divergence time estimation using the complete mitogenome. The level of variation was ~4%, and the time of divergence of the KLNP population and the other Indian populations was about 0.22 Mya, i.e., during the last glaciation periods of the Late Pleistocene/Early Holocene. The KLNP haplotypes of the control region were shared with the Southeast Asian subspecies, A. p. annamiticus. The results of the investigations of the microsatellite loci supported the mtDNA results unambiguously. Two genetically distinct lineages are found in India: one is found from the Terai Arc to Assam (A. p. porcinus) and the other in Manipur (A. p. annamiticus). The genetic diversity in KLNP was low and exhibited a higher degree of genetic differentiation compared with major Indian populations. The Bayesian skyline plots indicated that after a long phase of historic demographic stability, the populations of both the lineages of hog deer suffered pronounced declines during the period from ~800 years BP to 5000 years BP. In summary, our finding provided evidence that the KLNP population is probably a prime, isolated and sustaining stock of A. p. annamiticus and should be managed as evolutionarily significant units (ESUs).

Factors affecting forage selection by the endangered Eld's deer and hog deer in the floating meadows of Barak-Chindwin Basin of North-east India

Determination of resource based stocking density of wild ungulates living in the floating meadows of Keibul Lamjao National Park, India

Hog deer (Axis porcinus) are among the rarest animals in the world and are listed as "Endangered" on the International Union for Conservation of Nature’s Red List of Threatened Species. Protected areas in Nepal are home to large and stable populations of hog deer relative to other parts of hog deer range, but changes in predator abundance, human activities, and climate change could alter hog deer abundance. Reliable and efficient methods to assess hog deer population trends are therefore essential to monitor changes over time. Combined with data on population numbers, support from local communities towards hog deer conservation is important in identifying key threats and developing appropriate conservation strategies. Accordingly, this research integrated natural and social sciences to evaluate density estimation methods and community support for hog deer conservation within Shuklaphanta National Park (SNP), Nepal. Distance sampling along line transects is a common and relatively simple approach used to monitor hog deer populations in Nepal. Recent advances in technology, however, have enabled alternative approaches using data from remote-sensing cameras. Knowledge of the effectiveness of these two approaches could inform future monitoring efforts. Accordingly, I compared two methods for estimating the density of hog deer: distance sampling along line transects (n=17) and a random encounter model (REM) applied to data from remote-sensing cameras (n = 30). The density estimate produced by distance sampling (33.58 ± 8.48 individuals per km²) was more than double the estimate produced using the REM (12.95 ± 0.04 individuals per km²). Of the two methods, the estimate from the REM was more aligned with previous estimates of hog deer density. In addition, camera surveys facilitated the collection of data on multiple species, behaviour, and habitat use compared with line transects. However, despite requiring almost same amount of time for data collection, camera surveys proved to be more costly. These findings highlight the need for a comparison of these density estimation methods with established techniques such as mark-recapture or block counts, coupled with expanded survey efforts to assess the accuracy of estimates. To assess community support for hog deer conservation, I worked with research assistants to interview residents (n=30) from municipalities in the buffer zone surrounding SNP. Interview participants were knowledgeable about deer as a species group, but half of participants did not distinguish hog deer from other deer species. Most participants expressed positive attitudes towards hog deer conservation, especially in the context of promoting the local tourism economy around wildlife viewing. Participants explained that crop damage from deer has been largely mitigated by current management practices, such as fencing, but that additional fencing would further reduce conflicts. My findings revealed opportunities for collaboration between park managers and communities within the buffer zone, particularly around the development of the wildlife tourism industry and the design of management approaches such as fencing. Combined, my research provides guidance for future research and education efforts targeted at hog deer conservation.

Plant community structure of the floating meadows of a hypereutrophic wetland in the Indo-Burma Biodiversity Hotspot

The hog deer (Axis porcinus) is a small deer whose natural habitat is the wet or moist tall grasslands in South and Southeast Asia. Wild populations have dramatically decreased in recent decades. While wild hog deer were recently acknowledged to be extinct in China, a few captive populations have been maintained. In the present study, we successfully employed the restriction-site-associated DNA sequencing (RAD-seq) technique to generate a genome-wide profile of single-nucleotide polymorphisms (SNPs) in the captive population of hog deer from Chengdu Zoo, China (N = 11). Up to 4.7 million clean reads per sample were sequenced, and 11,155 SNPs and 8,247 haplotypes were simultaneously observed within more than 80% of sequenced individuals. In this population, the mean frequency of major alleles at each polymorphism site was 0.7903±0.0014, and the average nucleotide diversity (π) and inbreeding coefficient (FIS) were 0.3031±0.0015 and -0.0302±0.0062, respectively. Additionally, the Euclidean distance-based multidimensional scaling method revealed that the pairwise genetic relatedness was evenly distributed. However, the results of homologous searching by short reads did not provide any meaningful explanation of the phylogenetic relationship of hog deer, which should be further investigated. In conclusion, our results revealed current state of genetic diversity in this captive population of hog deer.Furthermore, these genome-wide SNPs would be useful for guiding the mating schedule to avoid sharp increase of inbreeding coefficient.

Context A wild population of non-native hog deer has established in the Gippsland region of Victoria, Australia, and there is particular concern about its impact on native vegetation in Wilsons Promontory National Park (WPNP). Since 2015, there has been annual culling of hog deer at WPNP to reduce deer abundances and impacts. Aims The aims of this study were to use a kinship approach based on genotyping to assess contemporary dispersal of hog deer across WPNP, by identifying close kin, to determine whether dispersal of deer into culled sites from unculled sites may affect the long-term success of management there. Differences in the dispersal of male and female hog deer were also investigated. Methods In total, 91 hog deer tissue samples were collected across WPNP and surrounding sites. Single nucleotide polymorphism (SNP) markers were sequenced, and a final dataset comprising 8275 SNPs was used for analysis. First-order, second-order, and intermediate relative pairs were identified, and the geographic distance between these pairs was assessed to determine inter-pair distances to infer dispersal. Spatial autocorrelation between male and female samples was evaluated to measure the effects of sex-biased dispersal. Key results Only seven second-order relative pairs were found across different sites, with a 30 km distance between the furthest pair observed. However, most inter-pair distances across sites were ~5–10 km. Analyses of sex-biased dispersal showed that movement by deer was not strongly influenced by one sex. Conclusions Although hog deer in WPNP are genetically similar, most relatives that were sampled were not widely dispersed. This suggests that there is limited dispersal of hog deer across this park. Implications Recolonisation of hog deer at culled sites via dispersal is likely to be infrequent in WPNP. Kinship analysis provides an effective method of assessing contemporary dispersal and could be applied to other species to assess fine-scale movement across landscapes.

In Australia, many species have been introduced that have since undergone drastic declines in their native range. One species of note is the hog deer (Axis porcinus) which was introduced in the 1860s to Victoria, Australia, and has since become endangered in its native range throughout South‐East Asia. There is increased interest in using non‐native populations as a source for genetic rescue; however, considerations need to be made of the genetic suitability of the non‐native population. Three mitochondrial markers and two nuclear markers were sequenced to assess the genetic variation of the Victorian population of hog deer, which identified that the Victorian population has hybrid origins with the closely related chital (Axis axis), a species that is no longer present in the wild in Victoria. In addition, the mitochondrial D‐loop region within the Victorian hog deer is monomorphic, demonstrating that mitochondrial genetic diversity is very low within this population. This study is the first to report of long‐term persistence of hog deer and chital hybrids in a wild setting, and the continual survival of this population suggests that hybrids of these two species are fertile. Despite the newly discovered hybrid status in Victorian hog deer, this population may still be beneficial for future translocations within the native range. However, more in‐depth analysis of genetic diversity within the Victorian hog deer population and investigation of hybridization rates within the native range are necessary before translocations are attempted.

Hog deer (Axis porcinus) is one of the least studied animal species in Pakistan. It belongs to Order Artiodactyla and the family Cervidae. IUCN classified Axis porcinus as an endangered species in 2008. The present study was conducted to investigate the effects of varying environmental exposure, genders, and seasonal changes on captive hog deer (A. porcinus) at Lahore Zoo, Safari Zoo, and Jallo Park in Lahore, Pakistan. Non-invasive techniques were used to monitor stress levels in hog deer. For sample collection, four definite months belonging to two seasons’ winter and summer were considered. A total of 48 urine and faecal samples were collected from both male and female hog deer. Seasonal fluctuations have been found to have a significant impact on faecal and urinary cortisol levels. Higher cortisol levels were found in both male and female hog deer in the summer season at all three visited sites. Fluctuations in environmental exposure at three research sites had a significant impact on faecal and urinary cortisol levels. Higher levels of cortisol were found in both male and female hog deer at Jallo park and Lahore Zoo, as compared to Safari Zoo. It was concluded that lower cortisol levels at Safari Zoo might be due to better environmental conditions and more flexible enclosure size and interaction of various species of deer. Temperature affected hog deer cortisol levels in summer, as higher levels were measured in summer compared to winter. In addition, sex did not predict any stress levels in hog deer. It has been suggested that a large enclosure size can control levels of cortisol in hog deer.

Endozoochory by exotic mammalian herbivores could modify vegetation composition by facilitating the dispersal and establishment of exotic and native plant species. We examined the potential for endozoochoric dispersal of native and exotic plants by exotic hog deer (Axis porcinus) in south-eastern Australia. We quantified the germinable seed content of hog deer faecal pellets collected in five vegetation types within a 10,500-ha study area that was representative of their Australian range. Twenty exotic and 22 native species germinated from hog deer faecal pellets and significantly more native species germinated compared to exotic species. Seedlings of the encroaching native shrub Acacia longifolia var. sophorae emerged, but no native trees emerged and the percentage of grasses that germinated was low (11%). The species composition of germinants was similar among the five vegetation types. We estimated that the hog deer population in our study area could potentially disperse >130,000 viable seeds daily. Our study shows how an exotic mammal can disperse seeds from both native and invasive plants and highlights the need for endozoochory to be considered more widely in studies assessing the impacts of exotic mammals on plant communities.

Hog deer were introduced to Australia in the 1860s, where they have spread across the Gippsland region of Victoria. Due to its status as an introduced species and an important game animal within Victoria, management of the species is complex. Given this complexity, genetic studies can provide important information regarding population structure and diversity which can assist in controlling problematic populations of hog deer, while also ensuring viable game stock in sites managed as game reserves. The aim of this study was to investigate the population genetic structure and diversity of the Victorian hog deer 150 years after introduction using short tandem repeats (STRs). Hog deer samples were collected across 15 sites of differing management regimes in the Gippsland region of Victoria and genotyped for 13 polymorphic STR loci. Up to four distinct genetic clusters were identified across the sites sampled, suggesting that despite low observed genetic diversity, population structure is present across their range. It was also possible to detect evidence of recent translocations among populations. This study suggests that the presence of distinct genetic clusters may enable management of separate genetic units, considering invasive species and game management objectives.

The unprecedented political and economic reforms taking place in Myanmar offer new opportunities for biodiversity conservation. They also bring new challenges in the form of rapidly growing extractive industry and agriculture sectors that have been weakly regulated and are often unsustainable. The Endangered hog deer Axis porcinus epitomizes many of these conservation challenges, and those facing most deer species in the Indo–Burma hotspot. The hog deer has disappeared from large parts of its range as a result of overhunting and intense conversion of its floodplain grassland habitat for agriculture. We report on a population of hog deer that was discovered in the Indawgyi landscape in central Myanmar in 2012. We conducted the first rigorous assessment of a hog deer population in Myanmar using an occupancy sampling protocol, tested the protocol's robustness using a power analysis, and present the results to guide management intervention. The results from our study site revealed widespread occurrence of the species, with high precision. The population map was then used to inform the development of a conservation management zone within a UNESCO Man and Biosphere Reserve around Indawgyi Lake. The importance of this population for the status of the hog deer in Myanmar remains unknown because documentation of the species has been sparse. Our survey protocol could make a significant contribution to addressing this knowledge gap and setting an informed agenda for conservation of the hog deer both nationally and more widely across the Indo-Burma hotspot.

The hog deer (Axis porcinus) is an endangered cervid with drastic population declines. There are 2 recognized subspecies of hog deer: A. p. porcinus, ranging from Punjab Province in Pakistan, Nepal, and the Northern part of India to Myanmar, and A. p. annamiticus found in Indo-China, Thailand, Laos, Cambodia, and Vietnam. The current geographic range of A. p. annamiticus is still ambiguous. We analyzed variation in the mitochondrial DNA control region (mtDNA CR) to investigate the intra-species structure, differentiation, and demographic history of hog deer from Cambodia (Kratie Province), which we compared with the populations from India and Thailand. We also generated divergence time estimates using a concatenated dataset of complete Cyt b and partial CR. The CR data showed that Cambodian hog deer are genetically differentiated from the mainland Indian and Thai populations, forming a distinct basal clade. The time of divergence indicates that the Cambodian lineage split from the other 2 hog deer lineages around 0.51 Mya, during the Late Pleistocene. The results also suggest strong phylogeographic structure among hog deer: lineage A extends from Terai Arc (foothills of the Himalayas) to Assam, India (A. p. porcinus), lineage B from Manipur, India to Thailand (A. p. annamiticus), and lineage C is only known from Kratie Province, Cambodia. Lineage A exhibited a higher level of genetic diversity than lineages B and C, with recent demographic stability. Thus, the hog deer population in Kratie Province appears to be a distinct lineage that should be treated as an evolutionarily significant unit.

Hog Deer (Axis porcinus), the rarest of Sri Lanka’s four deer species, is listed as Critically Endangered (CR) in the 2012 National Red List of Sri Lanka. Despite its status, the species has been studied very little within the country. This study, therefore, seeks to evaluate the current conservation status of the Hog Deer in the Galle District. The research was conducted over the course of one year, from December 1st, 2021 to December 1st, 2022. A total of 246 randomly selected participants from seven divisional secretariats in the Galle District were surveyed using a questionnaire. Recent observations recorded 306 adult Hog Deer and 22 fawns. Over a span of one and a half years, 27 injured and disabled Hog Deer were brought to the Hiyare Biodiversity Education and Research Center. They are restricted to human-modified landscapes, which they utilize on a seasonal basis. The survey documented 175 confirmed Hog Deer locations. Their habitat is limited to a small region in the Galle District, between the Bentota and Gin rivers. Hog Deer is known to cause damage to three crop types: Cinnamomum verum, Oryza sativa, and various vegetables, with Cinnamomum verum being the most frequently damaged plant. However, both the questionnaire survey and crop damage assessments indicated that the damage to cinnamon was minimal. The Hog Deer face significant threats within their range in the Galle District. Therefore, community-based conservation programs, along with institutional involvement, are essential for ensuring the survival of this charismatic species in its last remaining stronghold.

The hog deer (Axis porcinus), an Endangered grassland specialist of South and Southeast Asia, occurs at low densities across much of its range due to habitat loss and fragmentation. In Corbett Tiger Reserve, populations are mainly restricted to isolated alluvial grasslands, with major declines following the submergence of prime habitat by the Kalagarh Dam in 1974. To document distribution and provide rapid count indices, a three-day reconnaissance survey (22-24 May 2025) was undertaken in all 12 forest ranges, covering 141 beats during peak activity hours (06:00-10:00 am). Direct sighting counts were made daily, with the highest tally used as an index of relative abundance. Hog deer were recorded in only four ranges, indicating a restricted distribution. A total of 189 individuals were recorded, with observations expressed as relative abundance indices (CTR 0.15 ind/km²; Dhikala 2.31 ind/ km²). Dhikala accounted for 175 individuals, concentrated in the Dhikala Chaur and Jalashay beats, underscoring the role of high-quality alluvial grasslands and perennial water. As a rapid count without detection correction, findings represent indices rather than true population estimates. Results highlight the need for grassland management, invasive species control, and habitat connectivity to ensure long-term conservation of hog deer in CTR.