Delivery of tumor suppressive miRNA to triple negative breast cancer by targeting cationic liposome

Abstract

Objective To investigate the effect of delivering specific tumor suppressor genes miRNA in triple negative breast cancer (TNBC). Methods Film dispersion method was utilized to prepare for the new-pattern hyaluronic acid (HA)-targeted cationic liposome (HA-CLPs). The physicochemical property, such as the representation of the size and zeta-potential of CLPs nanoparticles, was assessed and then the nanoparticles were modified with HA. Confocal and flow cytometry (FCM) was applied to assess targeting capacity, while EdU cell proliferation test and cell wound scratch assay to assess biological effects of HA-CLPs. Results Nanoparticles of CLPs were of good dispersibility and shaped as homogeneous form of hollow globe. The average size of CLPs was (180.6±3.4) nm and the average zeta-potential was (43.4±2.8) mV. The average size of HA-targeted nanoparticles and miRNA-loaded HA-targeted nanoparticles was (236.65±6.9) nm and (205.6±2.2) nm. The average zeta-potential of HA-targeted nanoparticles and miRNA-loaded HA-targeted nanoparticles was (29.1±5.4) mV and (11.2±1.1) mV. Confocal and FCM verified the targeting capacity of HA-CLPs to deliver miR-205 around the MDA-MB-231 cell nucleus observably. EdU cell proliferation test and cell wound scratch assay proved that miR-205 and miR-34a sent into MDA-MB-231 cell had the ability to inhibit cell proliferation and migration. Conclusion Targeted cationic liposome has specific targeting ability towards MDA-MB-231 cell, and the delivery of miR-205 and miR-34a plays a part in the proliferation and migration inhibition of MDA-MB-231. Key words: Liposomes; Cations; Hyaluronic acid; Breast neoplasms