Abstract
The present study aimed to add value to cotton waste biomass using a more eco-friendly process, EnZolv which delignifies cotton stalk and cotton ginning mill waste. A maximum delignification of 68.68% and 65.51% was obtained using pre-optimized EnZolv parameters in cotton stalk (CS) and ginning mill waste (GMW), respectively. Optimized EnZolv process removed 78.68% of lignin in CS using Response Surface Methodology (RSM) in Box-Behnken design at 0% moisture content, 50 U laccase g−1 of biomass, 5 h incubation time, 50 ⁰C incubation temperature, and 150 rpm shaking speed. Similarly, RSM-based delignification of 70.53% in GMW was achieved under the optimized EnZolv conditions of 98.75 % moisture content, 41.59 U laccase g−1 of biomass, 9.3 h incubation time, 46.15 ⁰C incubation temperature, and 150 rpm shaking speed.
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