Abstract
Wheat leaves greened under intermittent illumination, which are devoid of the water-splitting activity in photosynthesis showed no fluorescence variation during actinic illumination and very weak delayed light emission after the illumination. Exposure of such leaves to continuous red light induced remarkable enhancement of delayed emission as well as variable fluorescence. Logarithmic recording of the induced emission intensity against time showed a curve with a shoulder, which indicated at least two emission components with different decay or delay constants. The intensification of delayed emission and fluorescence variation proceeded in parallel with the increase of the Hill activity in the early stage of activation by continuous light. It was suggested that the measurement of delayed emission from intact leaves as total photon counts is an accurate and rapid method to follow the process of photoactivation of the latent water-splitting sites in intermittently illuminated (ItI) leaves without isolating chloroplasts for activity measurements.
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