Abstract
Abstract In the Vicia faba-Rhizobium leguminosarum symbiosis defence reactions are established by a variable phytoalexin concentration in the nodules. No differences were observed between effective (fix+) and ineffective (fix- ) nodules. In nodules of field grown plants the average phytoalexin concentration was about 3 times higher than that from sterile pot-cultured plants infected with different Rhizobium leguminosarum strains. A great variability of phytoalexin accumulation in nodules of single plants was observed. In field grown plants about 60% of the nodules of each plant had very low levels (< 50 µg wyerone g nodule fresh weight-1) the other 40% had levels between 100 and 600 µig w yerone-g nodule fresh weight-1 indicating different defence reactions in the nodules distributed over the root system of one plant. Phytoalexin accumulation was more affected by the cultivar of the host plant than by the infecting Rhizobium strain. Two inbred lines had the lowest concentration in nodules
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