Abstract
X-ray-amorphous calcium phosphate (ACP) spheres were synthesized in a simulated/synthetic body fluid (SBF) solution heated to 55 to 70 °C under constant stirring at 850 rpm. The specific SBF solution (Lac–SBF) was buffered by using Na-l-lactate and lactic acid, and did not contain any Tris or Hepes. The Lac–SBF solution of this study flawlessly matched the concentrations of the inorganic electrolyte ions of the human blood plasma. The monodisperse ACP spheres synthesized at 55 °C were 245 nm in diameter when the Lac–SBF solution contained 67 mg/L gelatin. Samples were characterized by powder X-ray diffraction, Fourier-transform infrared spectroscopy, scanning electron microscopy and inductively-coupled atomic emission spectroscopy.
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