Abstract
In this study, the role of rho-kinase activity in the modulation of vascular contractility induced by hemin, a heme oxygenase inducer, was investigated. Aortic rings from Wistar rats were incubated in physiological saline solution (PSS) containing hemin at 10-(4) M for six hours then contracted with phenylephrine, and a dose-response curve was established. The effect of Y-27632, a rho-kinase inhibitor, on the relaxation of the pre-contracted aortic rings was then studied. Incubation of the aortic rings in hemin induced an increased expression of heme oxygenase 1 (HO-1). A reduction in the contractile force of aortic rings incubated in hemin was observed in response to phenylephrine. Y-27632 at a concentration of 10-(6) M induced a 36% relaxation of the control aortic rings but only a 20% relaxation in aortic rings treated with hemin. These data suggest that the decreased vascular contractility induced by hemin could, in part, result from an inhibition of rho-kinase activity.
Highlights
Aortic rings from Wistar rats were incubated in physiological saline solution (PSS) containing hemin at 10-4 M for six hours contracted with phenylephrine, and a dose-response curve was established
Cumulative concentrations of phenylephrine induced a concentration-dependent increase in the contraction of aortic rings
In the control aortic rings, the magnitude of the relaxation at 3 × 10-7 M of Y-27632 was 36% of the contraction induced by 10-6 M of phenylephrine
Summary
The role of rho-kinase activity in the modulation of vascular contractility induced by hemin, a heme oxygenase inducer, was investigated. Methods: Aortic rings from Wistar rats were incubated in physiological saline solution (PSS) containing hemin at 10-4 M for six hours contracted with phenylephrine, and a dose-response curve was established. The effect of Y-27632, a rho-kinase inhibitor, on the relaxation of the pre-contracted aortic rings was studied. Results: Incubation of the aortic rings in hemin induced an increased expression of heme oxygenase 1 (HO-1). A reduction in the contractile force of aortic rings incubated in hemin was observed in response to phenylephrine. Conclusion: These data suggest that the decreased vascular contractility induced by hemin could, in part, result from an inhibition of rho-kinase activity
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