Abstract
The present of chlorophyll is not expected in the isolation process of plant active constituent. Electrocoagulation is a potential dechlorophyllation method. This research aims to know the effectivity of electrocoagulation in the dechlorophyllation process of Cosmos caudatus Kunth., Morinda citrifolia, and Mangifera indica L. leaves methanolic extract as well as the effect to the total phenolic content. Electrocoagulation are performed using copper, silver, aluminum and iron plates as the electrode. Dechlorophyllation by extraction using n-hexane is performed as reference. The % absorbance of chlorophyll and % of total phenolic content of dechlorophyllated samples are measured spectrophotometrically. Electrocoagulation process reduces % absorbance of chlorophyll in Cosmos caudatus Kunth., Morinda citrifolia, and Mangifera indica L. leaves methanolic extract. The more the duration of electrocoagulaton process the lower the % absorbance of chlorophyll obtained. % absorbance of chlorophyll of the electrocoagulated extract according to paired t-test (P=0.95) are significantly different with the previous. One way ANOVA continued with LSD (P=0.95) shows that the % absorbance of chlorophyll from the electrocoagulated extract are significantly different with those extracted with n-hexane. Electrocoagulation process reduces total phenolic content along with duration of electrocoagulation.
Highlights
The present of chlorophyll is not expected in the isolation process of plant active constituent
This research aims to know the effectivity of electrocoagulation in the dechlorophyllation process
Dechlorophyllation by extraction using n-hexane is performed as reference
Summary
Keanekaragaman hayati Indonesia memiliki potensi besar dalam mendukung pengembangan obat yang berasal dari tanaman. Beberapa bagian tanaman yang berwarna hijau mengandung pigmen yang berupa klorofil, sehingga proses deklorofilasi dari ekstrak yang berasal dari bagian tanaman tersebut menjadi penting untuk dilakukan (Jumpatong dkk., 2006). Metode ini memiliki potensi untuk dikembangkan dalam usaha penghilangan klorofil dari ekstrak tanaman karena murah, cepat, dan ramah lingkungan. Penelitian ini diaplikasikan pada beberapa spesies tanaman yang mengandung klorofil dan berdasarkan beberapa penelitian sebelumnya telah terbukti memiliki khasiat terhadap kesehatan manusia yaitu kenikir (Cosmos caudatus Kunth.), mengkudu (Morinda citrifolia), dan mangga (Mangifera indica L.). Deklorofilasi dengan cara elektrokoagulasi dilakukan terhadap ekstrak metanolik dari daun tanaman tersebut dengan menggunakan berbagai macam elektroda yaitu tembaga, perak, aluminium dan besi. Penelitian ini diharapkan dapat memberikan informasi mengenai manfaat elektrokoagulasi dalam proses deklorofilasi ekstrak metanolik dan mengetahui pengaruhnya terhadap kandungan senyawa fenolik untuk keperluan isolasi senyawa aktif baik untuk tujuan identifikasi maupun produksi skala besar
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
