Abstract
The retention of large molecules, such as peptides and proteins, is strongly dependent on mobile-phase composition and temperature. Consequently, the retention times (tR) of these molecules often fluctuate from one separation to the next. In two-dimensional liquid chromatography (2D-LC), this fluctuation can be challenging to deal with, especially if only one or a few specific peaks in the first dimension (1D) separation are targeted for further separation. In this installment, we describe instrumental concepts that are useful for coping with this challenge.
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