Abstract
This dataset reports on the analysis of mouse hippocampus by LC–MS/MS, from mice fed a diet that was either deficient in n-3 FA (n-3 Def) or sufficient in n-3 FA (n-3 Adq). Label free quantitative (LFQ) analysis of the mass spectrometry data identified 1008 quantifiable proteins, 115 of which were found to be differentially expressed between the two dietary groups (n=8 per group). This data article refers to the research article “Omega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus” (English et al., 2013 [1]), in which a more comprehensive interpretation and analysis of the data is given.
Highlights
This dataset reports on the analysis of mouse hippocampus by LC– MS/MS, from mice fed a diet that was either deficient in n-3 FA (n3 Def) or sufficient in n-3 FA (n-3 Adq)
Label free quantitative (LFQ) analysis of the mass spectrometry data identified 1008 quantifiable proteins, 115 of which were found to be differentially expressed between the two dietary groups (n 1⁄4 8 per group)
This data article refers to the research article “Omega-3 fatty acid deficiency disrupts endocytosis, neuritogenesis, and mitochondrial protein pathways in the mouse hippocampus” (English et al, 2013 [1]), in which a more comprehensive interpretation and analysis of the data is given
Summary
LC–MS/MS was performed on an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific) coupled to an Ultimate 3000 RSLC Ultra High Pressure Liquid Chromatography system (Thermo Scientific Dionex). Raw MS/MS files (PRIDE archive), supplementary descriptive tables (Excel) Omega-3 Fatty acid deficient diet. Digested peptides were run in triplicate on an LTQ Orbitrap XL mass spectrometer. Label-free quantitation on the mass spectrometer data files was performed with MaxQuant. Mass spectrometry data files (.RAW), MaxQuant LFQ output (.txt) and Perseus statistical analysis (.txt) have been uploaded to ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD001277. Provides a valuable reference for future characterisation studies of brain proteins that are sensitive to changes in dietary omega-3 fatty acids
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