Abstract
The lacrimal gland is responsible for tear synthesis and secretion, and is derived from the epithelia of ocular surface and generated by branching morphogenesis. The dataset presented in this article is to support the research results of the effect of chitosan biomaterials on facilitating the structure formation of the lacrimal gland by regulating temporospatial dynamics of morphogen. The embryonic lacrimal gland explants were used as the standard experimental model for investigating lacrimal gland branching morphogenesis. Chitosan biomaterials promoted lacrimal gland branching with a dose-dependent effect. It helped in vivo binding of hepatocyte growth factor (HGF) related molecules in the epithelial-mesenchymal boundary of emerging epithelial branches. When mitogen-activated protein kinase (MAPK) or protein kinase B (Akt/PKB) inhibitors applied, the chitosan effects reduced. Nonetheless, the ratios of MAPK and Akt/PKB phosphorylation were still greater in the chitosan group than the control. The data demonstrated here confirm the essential role of HGF-signaling in chitosan-promoted structure formation of the lacrimal gland.
Highlights
Data supporting regulating temporospatial dynamics of morphogen for structure formation of the lacrimal gland by chitosan biomaterials
The lacrimal gland is responsible for tear synthesis and secretion, and is derived from the epithelia of ocular surface and generated by branching morphogenesis
The dataset presented in this article is to support the research results of the effect of chitosan biomaterials on facilitating the structure formation of the lacrimal gland by regulating temporospatial dynamics of morphogen
Summary
An ex vivo culture of the embryonic lacrimal gland explants was used. The ligands and receptors of HGF-related molecules were tested. The lacrimal gland explants were cultured in a chitosan-containing system to factors induce branching morphogenesis. The effect of chitosan on branching of the lacrimal gland explants was deterfeatures mined by imaging and quantitative analyses. The data allow other researchers to investigate the effect of biomaterial using the explants of embryonic lacrimal glands as an experimental model. The data reveals the morphogenetic effects of chitosan in facilitating lacrimal gland structure formation. The chitosan-mediated morphogenetic effects on lacrimal gland explants originated from increasing expression and binding of hepatocyte growth factor (HGF) related molecules
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