Abstract
Nuclear factor-kappaB (NF-κB) repressing factor (NKRF) specifically inhibits the transcriptional activity of NF-κB protein. The PmNKRF cDNA is composed of 757 amino acid residues. Alignment analysis revealed that the G-patch and R3H domains are conserved in different organisms. We aimed to analyse red sea bream NKRF (PmNKRF) gene expression after infection with pathogens [Streptococcus iniae or red sea bream iridovirus (RSIV)] and in healthy individuals. In healthy individuals, PmNKRF was ubiquitously expressed in all 12 tested tissues, predominantly in the head kidney and spleen. Expression of PmNKRF was significantly up-regulated in the gills, kidney, liver and spleen after RSIV infection. After S. iniae infection, PmNKRF expression was significantly down-regulated in the gills and significantly up-regulated in the kidney, liver and spleen.
Highlights
PmNKRF cDNA is composed of 757 amino acid residues
Alignment analysis revealed that the G-patch and R3H domains are conserved in different organisms
After S. iniae infection, PmNKRF expression was significantly down-regulated in the gills and significantly up-regulated in the kidney, liver and spleen
Summary
An ORF containing the sequence of PmNKRF was identified from the liver samples from bacteria infected red sea bream by NGS analysis. Sanger sequencing was performed to confirm the integrity of. Based on amino acid sequences, the characteristic domain was predicted using the Expert Protein Analysis System PROSITE Scan tool (http://prosite.expasy.org). Multiple sequence alignment was performed with the GENETYX software version 8.0 (SDC Software Development, Japan). Experimental animal and microbes Healthy red sea bream (weight: 173.2 ± 31.1 g, body length: 22.4 ± 0.9 cm) were obtained from the Gyeongsangnam-do Fisheries Resources Research Institute (Tongyeong, Republic of Korea), maintained. Bacteria were cultured in brain heart infusion medium at 27 C
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