Abstract
This paper contains original data supporting the antibacterial activities of Gallium (Ga3+)-doped pro-osteointegrative titanium alloys, obtained via Anodic Spark Deposition (ASD), as described in “The effect of silver or gallium doped titanium against the multidrug resistant Acinetobacter baumannii” (Cochis et al. 2016) [1].In this article we included an indirect cytocompatibility evaluation towards Saos2 human osteoblasts and extended the microbial evaluation of the Ga3+ enriched titanium surfaces against the biofilm former Escherichia coli and Staphylococcus epidermidis strains. Cell viability was assayed by the Alamar Blue test, while bacterial viability was evaluated by the metabolic colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Finally biofilm morphology was analyzed by Scanning Electron Microscopy (SEM). Data regarding Ga3+ activity were compared to Silver.
Highlights
This paper contains original data supporting the antibacterial activities of Gallium (Ga3 þ )-doped pro-osteointegrative titanium alloys, obtained via Anodic Spark Deposition (ASD), as described in “The effect of silver or gallium doped titanium against the multidrug resistant Acinetobacter baumannii” (Cochis et al 2016) [1]
Graphics, Scanning Electron Microscopy (SEM) images Viability via colorimetric assay evaluated by Optical density by spectrophotometer (Tecan Genius Plus; Tecan Italia, Cernusco Sul Naviglio, Italy); images by SEM (Zeiss EVO-MA10; Carl Zeiss, Oberkochen, Germany) Analyzed: means 7 standard deviations and statistics Grade II titanium disks were enriched with silicon, calcium, phosphorous and sodium using the Anodic Spark Deposition technique to obtain biomimetic surfaces
Images of the contaminated surfaces were obtained by SEM Saos2 human osteoblasts were purchased from European Collection of Cell Cultures (ECACC 89050205, distributed by Sigma-Aldrich, Milan, Italy); E. coli PHL628 and S. epidermidis RP62A isolates were kindly provided by the Universities of Pavia (Italy) and Dublin (Ireland), respectively Data are available in this article
Summary
Graphics (histograms), SEM images Viability via colorimetric assay evaluated by Optical density by spectrophotometer (Tecan Genius Plus; Tecan Italia, Cernusco Sul Naviglio, Italy); images by SEM (Zeiss EVO-MA10; Carl Zeiss, Oberkochen, Germany) Analyzed: means 7 standard deviations and statistics Grade II titanium disks were enriched with silicon, calcium, phosphorous and sodium using the Anodic Spark Deposition technique to obtain biomimetic surfaces. Images of the contaminated surfaces were obtained by SEM Saos human osteoblasts were purchased from European Collection of Cell Cultures (ECACC 89050205, distributed by Sigma-Aldrich, Milan, Italy); E. coli PHL628 and S. epidermidis RP62A isolates were kindly provided by the Universities of Pavia (Italy) and Dublin (Ireland), respectively Data are available in this article. Anodic Spark Deposition (ASD) is a novel suitable technique to apply biomimetic treatments to titanium surfaces. The data presented here provide new insights on Gallium and silver as useful antibacterial biomimetic treatments for pro-osteointegrative titanium based surfaces. We provide new data regarding the cytocompatibility (Supplementary Fig. 1) of electrochemically modified gallium or silver doped titanium alloys by Anodic Spark deposition and their antibacterial ability in counteracting biofilm formation produced by Escherichia coli and Staphylococcus epidermidis We provide new data regarding the cytocompatibility (Supplementary Fig. 1) of electrochemically modified gallium or silver doped titanium alloys by Anodic Spark deposition and their antibacterial ability in counteracting biofilm formation produced by Escherichia coli and Staphylococcus epidermidis (Supplementary Figs. 2 and 3)
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