Abstract
This study established the decimal reduction times (D values) of a composite inoculum of Salmonella enterica serotypes Typhimurium (American Type Culture Collection 13311 and E 1366) and Enteritidis (BIOTECH 1963) in Philippine native orange juice (pH 3.0, 0.84 % citric acid, 8.4°Brix). Acid-adapted cells (10 log10 cfu/g) were heated to 50–70C for 0–1.5 min and recovered by surface plating onto a selective (bismuth sulfite agar) and a nonselective medium (nutrient agar [NA]). Results showed that in both enumerating media and in all heating temperatures, survivor populations significantly decreased (P 0.05). The D values ranged from 0.16 to 0.40 min. PRACTICAL APPLICATIONS This study established the thermal inactivation rates of a composite Salmonella spp. inoculum in a Philippine orange juice medium. Salmonella is a target organism pertinent in the assuring citrus juices. Hence, appropriate thermal process schedules for citrus juices with similar physicochemical properties as the test medium, against the test organisms may be calculated using the results obtained from this study.
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