Abstract
D-amino acid oxidase is present in the extra-embryonic membranes of the developing chick, and can be detected on the third day of incubation (37°) four days prior to its earliest appearance in the hepatic tissue and mesonephros. As the membranes grow, those which form the yolk sac continue to show enzymatic activity. After the sixth day the mesonephros and hepatic tissue both contain activity and on the thirteenth day of development the enzyme appears in the metanephros. Post-hatch, when the mesonephros disappears, the metanephros maintains a specific activity higher than the liver. Enzymatic activity persists in the yolk sac membranes until several days after hatch.
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