Abstract
Objective: To investigate the cytotoxic effect of ethanolic extract of the leaves of asam kandis (Garcinia cowa Roxb.) against T47D breast cancer cells. Methods: The cytotoxicity of ethanol extract was carried out by measuring the activity of mitochondrial dehydrogenase in living cells that have ability to convert dissolved MTT pale yellow to purple formazan product. The extract was added at various concentrations (0.1, 1, 10 and 100 μg/mL). The level of cytotoxicity was determined by calculating the IC50 value that was based on the percentage of the cell death after 24 hours treatment with the extract. Cell morphological changes were observed by using inverted microscope. Results: The IC50 value showed that ethanol extract of leaves of asam kandis could resist T47D breast cancer cells with IC50 6.13 ± 3.51 μg/mL. The statistic results proved that ethanol extract of the leaves of asam kandis could inhibit the growth of T47D breast cancer cells significantly at concentrations of 10 μg/mL and 100 μg/mL. Conclusion: The results suggest that ethanol extract of the leaves of asam kandis was potential source of herbal medicine for cancer-related ailments.
Highlights
Plants have beneficial activity in different type of diseases producing in human beings
In continuating of our studies on asam kandis (G. cowa),[3,8] we further investigated the cytotoxicity of the leaves of this species on T47D breast cancer cells line
The cytotoxicity effect of ethanol extract of leaves of asam kandis on T47D was evaluated by MTT assay
Summary
Plants have beneficial activity in different type of diseases producing in human beings. About 80% of the world’s populations still use the medicinal plants for their primary health care.[1] Many pharmaceutical drug discoveries originated from traditional folk medicine and its associated plant materials and bioactive secondary metabolites. Garcinia (Family Guttiferae) is a large genus of polygamous trees or shrubs distributed in tropical Asia, Africa, and Polynesia. Garcinia cowa Roxb., known asam kandis is a medium-sized tree with edible fruit which attains a height of about 30 m. Asam kandis has been used by Minang tribes for many purposes. The dry fruit of kandis is used as seasoning and in traditional folk medicine is used for treating nausea, constipation and ulcers.[2]
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