Cytotoxic and suppressor activities in patients with HTLV-I-associated myelopathy

Abstract

Since there are several immune abnormalities and autoimmune-like features in human T-cell lymphotropic virus type I (HTLV-I)-associated myelopathy/HTLV-I-associated tropical spastic paraparesis (HAM/TSP), we examined cytotoxic and suppressor cell functions in HAM/TSP. In this study, we assayed cell-mediated cytotoxicity of CD8 + T-cell lines established from cerebrospinal fluid lymphocytes of two patients with HAM and concanavalin A-induced suppressor cell activities of peripheral blood lymphocytes from five patients with HAM. Our study revealed that three of four CD8 + T-cell lines showed cytotoxic activities against autologous CD4 + T-cell lines infected with HTLV-I, and two of the three lines showed major histocompatibility complex class I-restricted cytotoxicity. We also demonstrated that the concanavalin A-induced suppressor function was not defective in HAM patients. Therefore, the immune abnormalities and autoimmune-like features observed in HAM/TSP may not result from defective cytotoxic or suppressor cell activities.