Abstract
Rutin is a flavanoid with many biological activities including antiallergic, anti-inflammatory, anti-proliferative and ant carcinogenic properties. Rutin along with Vitamin C and K combination are used as neutraceuticals in the treatment of Cancer, Haemorrhoids, Vit K deficiency, varicose veins, Wound healing, Scurvy e.t.c. The present study evaluates the anticancer effect of Rutin, Ascorbic acid and Menindione (RAM) combination against two cell lines, one cancer and one normal cell line using Hep G2 (Human liver carcinoma cells) and L929 (fibroblast) cells by MTT assay. Morphological changes after exposure with RAM were studied under phase contrast microscope in a dose dependent manner. RAM shows an IC 50 value of 47.99 µg/ml against Hep G2 and 23.612 against L929 respectively. This shows that the combination is more cytotoxic than promising anticancer activity which is contrary to the beneficial effects claimed by these formulations.
Highlights
The term neutraceuticals are derived from two words‟ Nutrient” and pharmaceuticals
The cells were maintained in Dulbecos modified Eagles medium (DMEM, Gibco, Invitrogen) which was supplemented with 10% FBS, L glutamine, sodium bicarbonate and antibiotic solution containing penicillin (100U/ml), Streptomycin (100U/ml) and amphotercin B (2.5 μg/ml) and kept at 37 °C in a humidified 5% CO2 incubator (NBS Eppendorf, Germany) and 95% air
Cytotoxic and anti proliferative effect of RAM was determined against L929 and Hep G2 cell lines by MTT assay
Summary
The term neutraceuticals are derived from two words‟ Nutrient” and pharmaceuticals. They differ from dietary supplements as they help in disease prevention and treatment [1,2]. Rutin is a flavanoid and is having many biological activities including antiallergic, anti-inflammatory, antiproliferative and anticarcinogenic properties. It is of natural origin and present in many fruits and vegetables [3]. Rutin along with Ascorbic acid (Vitamin C) and Menindione (Vitamin K) abbreviated as RAM, are used as neutraceuticals in the treatment of Cancer, Haemorrhoids, Vitamin K deficiency, Varicose veins, Wound healing, Scurvy e. The present study evaluates the anti cancer effect of this combination in HepG2 and LN29 Cell lines
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