Abstract
Objective: This study aimed to determine the content of phytochemical screening and cytotoxic activity indicated by the IC50 value of the ethanol extract of bajakah tampala stem.
 Methods: Phytochemical screening for extract consists of saponins with distilled water, steroids and terpenoids were determined with the reagent glacial acetic acid and sulphuric acid, tannin using reagent 10%, FeCl3, alkaloids determined with reagents Mayer, flavonoids using reagent HCl, Mg powder, and phenolic with using 2%. FeCl3. The WST-8 procedures were used to investigate the cytotoxic activity of the MCF-7 breast cancer cell type.
 Results: Based on the results showed that the ethanol extract of bajakah tampala stem has secondary metabolite content, namely the presence of saponins, steroids, tannins, alkaloids, flavonoids and phenolics. The results of the cytotoxic test of ethanol extract of bajakah tampala stem have cytotoxic activity with IC50 of 193.2 mg/ml, which is included in the moderately active category.
 Conclusion: In this study, the ethanol extract of bajakah tampala stem has secondary metabolite content and cytotoxic activity against MCF-7 breast cancer cells.
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