Abstract
Incubation of Achlya chromatin with antheridiol and the cytosol from male strain E87 increases the RNA synthetic capacity of the chromatin. Neither cytosol, nor antheridiol alone, nor mixtures of hormone with cytosol from the female strain 734 stimulated transcription of the chromatin. A temperature-dependent incubation of the steroid-cytosol complex and high ionic strength (0.05 – 0.12 M potassium chloride) was required for optimal hormone-cytosol stimulation of in vitro transcription. These results strongly suggest that complex hormonal control mechanisms similar to those found in animal systems exist in the primitive fungus Achlya.
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More From: Biochemical and Biophysical Research Communications
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