Abstract
How the crowded confines of a cell influence the assembly and organization of intracellular structures remains a mystery. While some cytoskeletal structures have been successfully formed in vitro from purified protein components, biochemical reconstitution outside the complex environment of the cell carries the inevitable risk of removing too much context and therefore sacrificing important physical constraints that guide the system's behavior. One such constraint is the size of cells. This talk will describe recent work investigating the effect of volumetric confinement on the assembly of mitotic spindles in Xenopus egg extract, as well as experimental methods for recreating physical constraints for in vitro reconstitutions. This work contributes to the growing view that cytoskeletal structures in cells are defined not only by their molecular components but also by their boundary conditions.
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