Abstract
13C-NMR spectroscopy was used to determine the level of cytoplasmic malate in maize root tips that exhibited different rates of malate synthesis. Intracellular malate was 13C-labeled at carbons 1 and 4 by perfusing root tips with 5 mM H 13CO 3 −. This labeling reflects the activities of phospho enolpyruvate carboxylase and malate dehydrogenase (production of [4- 13C]malate), and fumarase (scrambling of 13C-label between C1 and C4 of malate). In vivo 13C-NMR spectra contained a clearly resolved resonance from cytoplasmic [4- 13C]malate, while the resonance from cytoplasmic [1- 13C]malate overlapped with others. After 90 min of H 13CO 3 − treatment, 13C-labeling of organic acid pools and reached steady-state. Thereafter, the ratios [ 13 C] malate/[ 12 C + 13 C] malate and [1- 13C]malate/[4- 13C]malate in tissue extracts remained constant; evidence is presented that these ratios were the same for both cytoplasmic and total cellular malate. Hence, the intensity of the cytoplasmic [4- 13C]malate signal was proportional to the amount of cytoplasmic malate in root tips. Potassium sulfate stimulated malate synthesis in maize root tips, relative to root tips perfused with HCO 3 − alone; total cellular malate doubled after approx. 1 h of 5 mM K 2SO 4-treatment. Cytoplasmic malate increased from approx. 3.5 mM to approx. 7.5 mM within 45 min of the onset of K 2SO 4-treatment, declining slightly thereafter. The possible effects of these changing cytoplasmic malate concentrations on the enzymes involved in malate metabolism are discussed.
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More From: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
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