Abstract
The degree of basophilia in the cytoplasm of Purkinje cells of 2 rabbits was examined by microspectrophotometry after staining with gallocyanin. No statistically significant differences in extinction values between the 2 rabbits and within the measured areas in the 2 cerebella was demonstrated. These findings and the examination of Purkinje cells stained with PAS-gallocyanin give no basis for the existence of 2 or more types of Purkinje cells. Therefore, the differences in staining intensity are not believed to be evidence of more than one type of cell. A subjective visual judging of the degree of basophilia has been attempted, but this method could not be used for accurate estimations. The uniform distribution of Purkinje cells in different stages of function is related to newer theories on the neurophysiological functions of the cerebellar cortex, and to the different subdivisions of cortex, based on anatomical and physiological investigations.
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