Abstract
The subcellular localization of NAD+‐dependent glutamate dehydrogenase (GDH; EC 1.4.1.4) in leaves of barley (Hordeum vulgare L. cv. Hassan) was studied during leaf senescence induced by detachment and incubation in the dark. GDH strongly increased in the cytoplasmic fraction isolated by differential centrifugation during senescence. It also showed a retarded and low increase in the mitochondrial fraction. No GDH was detected in the chloroplast fraction. The marker of the cytoplasmic fraction glucose‐6‐phosphate dehydrogenase (G‐6‐P dehydrogenase; EC 1.1.1.49) rapidly decreased after the induction of senescence. The effects of kinetin, gibberellic acid, abscisic acid and ethylene on the levels of GDH and G‐6‐P dehydrogenase were, in general, in agreement with the known hormonal effects on other senescence symptoms.
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