Abstract
Bacteria-free tobacco ( Nicotiana tabacum cv. Wisconsin #38) crown gall strains incited by Agrobacterium tumefaciens C58, 27, B6, CGIC, and AT4 have been analyzed for cytokinin content with the tobacco callus bioassay. All tumor strains contained high total levels of cytokinins ranging from 4–810 kinetin equivalents per kg fresh weight compared to 0.5 kinetin equivalents per kg for normal callus growing on medium with 0.1 μM N6-benzyladenine. Fractionation on a column of Sephadex LH-20 separated cytokinin activity from B6 tumors into a number of components among which ribosyl- trans -zeatin has been purified and characterized based on uv spectrum, biological activity and mass spectrum.
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