Cytokine removal on extracorporeal life support for treatment of acute endotoxemia: A randomized controlled animal study

Abstract

BackgroundWe prospectively evaluated the effectiveness of resin adsorption incorporated in an extracorporeal life support (ELS) circuit in an animal model of sepsis for removal of cytokines and prevention of hemodynamic deterioration during the treatment of septic shock. MethodsTwelve female landrace pigs were randomly assigned to two groups, a study group(n=6), treated with high-flow resin adsorption (300mL/min) and ELS, and a control group (n=6), treated only with ELS. Septic shock was induced by intravenous 0.02μg/kg/min infusion was of Escherichia coli lipopolysaccharide (LPS). Measurements were carried out in the study group at baseline, at the end of LPS injection(t0) at 30(t1), 60(t2), 90(t3) and 120min (t4) and 60min after stopping resin-adsorption (t5). In the control group measurements were performed at baseline (t0), t1 and only t2, as no control animal survived beyond this latter experimental timepoint. ResultsThe final population consisted of 9 animals, five in the study group and 4 in the control group. Plasma values of both tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) were reduced during resin-adsorption (t1–t4) while these mediators increased in controls undergoing ELS only. With a clearance of TNF-α of 15,233pg/min and IL-6 of 10,233pg/min, 79.2% of TNF-α and 95.3% of IL-6 produced were adsorbed. Systemic vascular resistance decreased significantly in both groups at t0. While it further was reduced during the control experiments at t1 and t2, it returned to normal in the study animals. Cardiac output increased at t0, t1 and t2 in the control experiments. In contrast, in study animals after a peak at t0, it returned to the baseline value and did not vary thereafter. ConclusionsCombined resin-adsorption and ELS improved hemodynamics resulting from effective removal of inflammatory mediators in a pig model of septic shock.