Cytokine regulation of glucose entry into the skeletal muscle of the rainbow trout (Oncorhynchus mykiss): role of tumour necrosis factor alpha (TNFα)

Abstract

Event Abstract Back to Event Cytokine regulation of glucose entry into the skeletal muscle of the rainbow trout (Oncorhynchus mykiss): role of tumour necrosis factor alpha (TNFα) Yoryia Vraskou1*, Nerea Roher2, Simon Mackenzie2 and Josep Planas1 1 University of Barcelona, Department of Physiology, Spain 2 Universitat Autonoma de Barcelona, Departamento de Biologia Cellular, Immunologia i Fisiologia Animal, Spain Tumour necrosis factor alpha (TNFα) is one of the most studied cytokines for its catabolic effects in target cells during excessive production. TNFα is known to have a direct action on skeletal muscle in mammals. However, little is known regarding the potential effects of cytokines on non-immune tissues, and particularly in skeletal muscle, in fish. The aim of this study was to investigate the effects of recombinant trout TNFα (rtTNFα), on skeletal muscle function in rainbow trout (Oncorhynchus mykiss). We used a primary cell culture of muscle cells from rainbow trout to show that rtTNFα stimulates glucose uptake in myoblasts and myotubes at concentrations that do not affect the viability of the cells and requiring de novo protein synthesis, as shown by the impairment of rtTNFα-stimulated glucose uptake by cycloheximide. With the use of specific inhibitors we show that rtTNFα-stimulated glucose uptake is mediated by the p38-MAPK, NF-kB and JNK pathways . Additionally, we provide evidence that the stimulatory effects of rtTNFα on glucose uptake in trout skeletal muscle cells may be caused, at least in part, by an increase in the amount of GLUT4 at the plasma membrane. Incubation of trout muscle cells with conditioned medium from LPS-stimulated trout macrophages stimulated glucose uptake. Our results indicate that recombinant as well as native trout TNFα directly stimulates glucose uptake in trout muscle cells and provides evidence, for the first time in non mammalian vertebrates, for a potential regulatory role of TNFα in skeletal muscle function. Keywords: comparative endocrinology Conference: 25th Conference of the European Comparative Endocrinologists, Pécs, Hungary, 31 Aug - 4 Sep, 2010. Presentation Type: Conference Presentation Topic: Comparative endocrinology Citation: Vraskou Y, Roher N, Mackenzie S and Planas J (2010). Cytokine regulation of glucose entry into the skeletal muscle of the rainbow trout (Oncorhynchus mykiss): role of tumour necrosis factor alpha (TNFα). Front. Endocrinol. Conference Abstract: 25th Conference of the European Comparative Endocrinologists. doi: 10.3389/conf.fendo.2010.01.00069 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Aug 2010; Published Online: 29 Aug 2010. * Correspondence: Dr. Yoryia Vraskou, University of Barcelona, Department of Physiology, Barcelona, Spain, yoryiavrascou@ub.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Yoryia Vraskou Nerea Roher Simon Mackenzie Josep Planas Google Yoryia Vraskou Nerea Roher Simon Mackenzie Josep Planas Google Scholar Yoryia Vraskou Nerea Roher Simon Mackenzie Josep Planas PubMed Yoryia Vraskou Nerea Roher Simon Mackenzie Josep Planas Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.