Abstract
Cytochrome P450 (P450 or CYP) monooxygenases play an important role in the oxidative metabolism of a variety of lipophilic compounds including secondary metabolites as well as foreign chemicals in higher plants. It was reported that CYP78A1 was preferentially expressed in developing inflorescences of Zea mays. In addition, we isolated a cDNA clone of the novel cytochrome P450 species CYP703A1 from Petunia flower buds. A high level of the transcript of cyp703A1 was found at the early stage of flower development. In order to analyze the enzyme functions of these P450 species, CYP78A1 and CYP703A1 were expressed in the yeast Saccharomyces cerevisiae AH22 cells under the control of alcohol dehydrogenase I promoter and terminator. The recombinant yeast microsomes containing each of the CYP703A1 and CYP78A1 hemoproteins were found to metabolize lauric acid to give hydroxylated metabolites. We will discuss the relationship between the metabolism of fatty acids and flowering in both dicot and monocot plants.
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