Abstract
Publisher Summary This chapter discusses the cystathionine β-Lyase from Escherichia coli (E. coli). Cystathionine β -lyase is an enzyme in methionine biosynthesis present in enteric microorganisms. The enzyme has broad substrate specificity capably of degrading L-cystine, L-cysteine, L-djenkolic acid, L-homolanthionine, and L-meso-lanthionine in addition to L-cystathionine. The chapter also updates a previous source for the enzyme from a Salmonella metA-15 mutant which overproduced the enzyme 7- fold over wild-type levels. By cloning the Esherichia colt metC gene on a ColE1 plasmid, and by transferring this plasmid into a regulation-deficient met J mutant, 100-fold overproduction of β -cystathionase above the wild- type E. coli level could be obtained. This strain (ATCC 37384) now represents the most enriched source for the enzyme that is available. The enzyme was assayed by measuring the rate of formation of free thioi groups by spontaneous disulfide interchange with 5,5'- dithiobis(2-nitrobenzoic acid) (DTNB), similar to the described procedure for γ-cystathionase.
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