Cyclophosphane effect on peripheral blood leukocytes and organs of immune system in experimental animals

Abstract

Event Abstract Back to Event Cyclophosphane effect on peripheral blood leukocytes and organs of immune system in experimental animals Anatoliy P. Godovalov1*, Olga V. Lebedinskaya2, Elena A. Lebedinskaya2, Dmitriy V. Suvorov2, Alexandra N. Razdobreeva2 and Andrey V. Khorinko2 1 Acad. E.A. Wagner Perm State Medical Academy, immunology, Russia 2 Acad. E.A. Wagner Perm State Medical Academy, histology, embriology and cytology, Russia The aim of investigation was the analysis of cyclophosphane (CP) effect on the immune system organs, on peripheral blood leukocytes in experimental animals. The study was conducted in 20 male rats (284±20 g). CP was administered intraperitoneally at a concentration of 100 mg/kg of body weight, 4 fold with 24h interval. Blood samples were taken from the tail vein to determine the total leukocyte count and leukocyte formula. Pathomorphological changes in bone marrow, thymus, spleen and lymph nodes were analyzed through 48 h after final CP injection. It was shown that leukocyte number reduced by 50% on the 2nd day after the first CP injection, and by 99% - in subsequent two days. After the final CP injection the recovery of initial leukocyte number was not detected. Following the first CP introduction there was noted statistically significant increase in segmented neutrophils that were sharply declined after the 2nd cytostatic injection and were not further recovered. The amount of monocytes and lymphocytes was lowered 24h after the first injection of cyclophosphane and subsequently continued to reduce. The alteration in the functional activity was determined in phagocytic cells. The statistically significant reduction in thymus, spleen and lymph node weight, was detected, as well as degenerative changes therein and decrease nucleated cells in bone marrow. Thus as a result of investigation the development of induced immunosuppression was revealed that involved the lowering in blood leukocyte count, alteration of their composition and functional activity, progression of degenerative changes in the organs of immune system. Keywords: cyclophosphane, Immunosuppression, degenerative changes, phagocytic cells, leukocyte formula Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Godovalov AP, Lebedinskaya OV, Lebedinskaya EA, Suvorov DV, Razdobreeva AN and Khorinko AV (2013). Cyclophosphane effect on peripheral blood leukocytes and organs of immune system in experimental animals. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00593 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Anatoliy P Godovalov, Acad. E.A. Wagner Perm State Medical Academy, immunology, Perm, Russia, AGodovalov@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Anatoliy P Godovalov Olga V Lebedinskaya Elena A Lebedinskaya Dmitriy V Suvorov Alexandra N Razdobreeva Andrey V Khorinko Google Anatoliy P Godovalov Olga V Lebedinskaya Elena A Lebedinskaya Dmitriy V Suvorov Alexandra N Razdobreeva Andrey V Khorinko Google Scholar Anatoliy P Godovalov Olga V Lebedinskaya Elena A Lebedinskaya Dmitriy V Suvorov Alexandra N Razdobreeva Andrey V Khorinko PubMed Anatoliy P Godovalov Olga V Lebedinskaya Elena A Lebedinskaya Dmitriy V Suvorov Alexandra N Razdobreeva Andrey V Khorinko Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.