Abstract
A microwave apparatus applied for radiation on the animal tissue in vitro as well as in vivo was designed and produced by us in cooperation with NEC Co. The temperature of Locke's solution in an organ bath of 5 ml and that of the mouse brain set in the microwave apparatus increased linearily with increasing the microwave intensity and irradiation time. When the Locke's solution and the mouse brain was irradiated by the microwave having an intensity of 2.0 kw for 2.0 sec, their temperatures increased up to about 90°C.Levels of cyclic AMP and cyclic GMP in the isolated rat papillary muscle, kept in Locke's solution and irradiated by the microwave (2.0kw) for 2.0 sec, were 4.075 and 2.098 pmol/mgprotein, respectively. When the papillary muscle was treated with aceton dry ice instead of the microwave radiation, those levels were 5.870 and 1.382pmol/mgprotein, respectively. Between the levels of cyclic AMP or cyclic GMP in the papillary muscles treated with microwave irradiation and those treated with aceton dry ice, there was no significant difference. When the rat papillary muscle driven by electrical stimulation in a frequency of 2 Hz for 30 min and irradiated by microwave (2.0kw) for 2.0 sec, levels of cyclic AMP and cyclic GMP in the muscle were 4.178and 0.310 pmol/mg-protein, respectivley.Levels of cyclic AMP and cyclic GMP in the brain of mouse irradiatedby microwave (2.0kw) for 2.0 sec were 6.494 and 0.772 pmol/mg. protein, respectively.
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