Abstract
Objective To investigate the role of chemokine receptor 4 (CXCR4) in the development of bladder cancer. Methods QPCR was used to detect the expression of CXCR4 mRNA in normal bladder tissues, bladder cancer tissues and bladder cancer cell lines SW780, 5637 and T24. MTT, colony formation, and transwell migration and invasion assays were performed in SW780 cells after treatment with the CXCR4 antagonist AMD3465. β-catenin, Wnt/β-catenin pathway targeted genes and epithelial-mesenchymal transition (EMT) related genes were detected by Western blot. The effect of AMD3465 on the growth of human bladder cancer SW780 cell xenografts was evaluated using a nude mice model. Results The expression of CXCR4 in bladder cancer and bladder cancer cell lines was significantly higher than that in normal bladder tissues. AMD3465 could effectively inhibit the proliferation, colony formation, migration, and invasion of SW780 cells, meanwhile, it significantly inhibited the expression of β-catenin and Wnt/β-catenin pathway targeted genes. The expression of EMT related gene E-cadherin was significantly up-regulated. Conclusions CXCR4 affects the development of bladder cancer by regulating Wnt/β-catenin signaling pathway. Key words: Urinary Bladder Neoplasms; Receptors, CXCR4; Cell Proliferation; Cell Movement
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