Abstract
The role of gene expression in regulating cuticle formation was examined in the second molt of Nippostrongylus brasiliensis. Worms were grown in formalin-killed Escherichia coliand actinomycin-D or α-amanitin were added at various times after isolation of eggs. These transcription inhibitors prevented molting when added to cultures before 96 h but cuticle formation was not inhibited when they were added at 96 and 102 h. Sucrose gradient analysis indicated the biosynthesis of 18S and 28S RNA at 90 h. Actinomycin-D inhibited the formation of that RNA but α-amanitin did not alter biosynthesis. The above experiments strongly suggest that messenger RNA specific for molting was synthesized at 90 h.
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