Abstract
Aeromonas sobria is opportunistic pathogen frequently found in environment and food. Interfering with its quorum sensing (QS) system could be a promising way to alleviate its virulence. In this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, PDI (Polymey Disperse Index), encapsulation efficiency and loading capacity were measured. The quorum sensing inhibitory effect of curcumin liposomes under sub-MIC (Minimum Inhibitory Concentration) on siderophore production, swimming and swarming motility, extracellular proteases, biofilm formation and AHLs (N-acylhomoserine lactones) production of A. sobria were also determined. The results showed that, the curcumin liposomes with high encapsulation capacity (84.51 ± 0.58%) were stable and homogeneous. QS-regulated phenotypes of the pathogen were significantly inhibited by curcumin liposomes. The in silico analysis revealed that the QS system of A. sobria may be inhibited by released curcumin from curcumin liposomes through interacting with the built LuxI type protein and blocking the production of AHLs.
Highlights
Aeromonas sobria is a Gram-negative, rod-shaped, facultative anaerobic, motile, and flagellated bacterium of the Aeromonadaceae family[1]
Blocking the bacterial quorum sensing (QS) system has been considered as an alternative of traditional antibiotics, for it would not induce the resistance of bacteria due to its distinctive antibacterial mechanism[8]
The results revealed that curcumin liposomes significantly decreased the biofilm formation in A. sobria
Summary
Aeromonas sobria is a Gram-negative, rod-shaped, facultative anaerobic, motile, and flagellated bacterium of the Aeromonadaceae family[1]. Quorum sensing is a cell-to-cell communication mechanism, allowing bacteria to monitor their population density by secreting signaling molecules or autoinducers[4]. The brominated furanones such as furanone C-30 have been reported to inhibit QS-regulated biofilm formation and production of virulence factors[14]. Curcumin has been shown to have inhibitory effect on the virulence, QS, and biofilm initiation of Pseudomonas aeruginosa PAO1 in the whole plant and animal models[20]. In the present study, the curcumin liposomes were prepared to study the inhibitory effect on quorum sensing system of A. sobria. The possible inhibitory mechanism was further analyzed by molecular docking techniques
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