Abstract
Oxidation of several exogenous substrates by cultured adult rat ventricular cardiac muscle cells has been assessed. Unlike freshly isolated cardiac muscle cells which oxidize glucose preferentially, the cultured cells more closely resemble metabolically the in situ heart and the isolated perfused heart, in that their preference for exogenous substrates is in the order of fatty acid > glucose. This switch in metabolic preference from glucose to fatty acid is complete within 12 h after placing freshly isolated cells in culture. Glucose oxidation is stimulated by insulin and isoproterenol and inhibited by β-hydroxybutyrate and octanoate. The adenylate cyclase system has also been examined in these cultured cells. Isoproterenol, norepinephrine and epinephrine stimulate the accumulation of cyclic adenosine 3:5-monophosphate (cyclic AMP) in a concentration-dependent manner. The order of potency is isoproterenol > norepinephrine ≊ epinephrine. This stimulation is potentiated by 1-isobutyl-3-methylxanthine and inhibited by 1-propranolol.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
