Abstract
Summary Induction of Arabidopsis thaliana (L.) Heynh. anther cultures on liquid and agar-solidified media was attempted. Anthers floated on stationary liquid medium produced greater numbers of calluses as compared with medium containing agar. Mean chromosome numbers were lower for liquid-grown tissue and maximum shoot regeneration rates were higher. The effects of auxin and cytokinin type and concentration on the success of anther cultures in liquid medium also were examined. The auxin 4-amino-3,5,6 trichloropicolinic acid (trade name «Tordon») enhanced callus induction rates and produced greater frequencies of haploid and haploid-derived shoots than did 2,4-dichlorophenoxyacetic acid (2,4-D), the standard auxin for A. thaliana . Naphthalene acetic acid was found to be less effective than 2,4-D as an auxin in the callus induction and similar results were obtained for N-6-benzyladenine as a substitute for kinetin.
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