Abstract
AbstractThe effects of sodium bicarbonate on freshwater green alga Neochloris oleoabundans were evaluated with respect to cell growth, cell morphology, and control of protozoa contamination under non‐sterile conditions co‐inoculated with protozoa pre‐cultures. While culture collapse occurred in the control and the cultures containing 40 mmol/L NaHCO3 due to protozoa contamination, the addition of 160 or 200 mmol/L NaHCO3 could completely inhibit the growth of microalgae‐preying protozoa and allow safe cultivation of N. oleoabundans for co‐incubation with protozoa in non‐sterile media. These results suggest that well‐formulated media containing appropriate amounts of dissolved inorganic carbon (DIC) may enable low‐cost cultivation of freshwater microalgae in non‐sterile open systems.
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