Abstract
The objective of this work was to identify growth-promoting bacteria isolated from Agaricus blazei and to evaluate their effect on mushroom mycelial growth and productivity. A total of 56 A. blazei-associated bacterial isolates were obtained from casing soil and identified by 16S rRNA gene sequencing. Bacteria were evaluated as to phosphate-solubilization ability, nitrogen-fixation capability, and secretion of cellulase. Superior isolates were tested for their to effect on A. blazei productivity, micelial growth, and on the contents of the polysaccharide-protein complex and of N, P, K, Ca, and Mg. Bacterial isolates were identified as actinobacteria (60%), firmicutes (20%), and proteobacteria (20%). Among them, ten isolates had phosphate-solubilization ability, eight showed nitrogen-fixation capability, and 12 isolates promoted A. blazei mycelium growth. Bacterial inoculation reduces time till harvest in up to 26 days, increases fresh mushroom yield up to 215%, and increases total polysaccharide-protein complex content twofold when compared to the non-inoculated control. The actinobacteria group is the predominant A. blazei-associated phylum.
Highlights
Plant root exudates influence the diversity and activity of rhizobacteria, and may play important roles in nutrient mobilization and plant growth (Kumar et al, 2012)
Bacterial count in A. blazei‐associated soil from triplicates was estimated at approximately 1.3x106 cfu g‐1
A total of 56 A. blazei‐associated bacteria isolates were identified in the present study (Table 2)
Summary
Plant root exudates influence the diversity and activity of rhizobacteria, and may play important roles in nutrient mobilization and plant growth (Kumar et al, 2012). Growth‐promoting rhizobacteria (PGPR) in the vicinity of plant root tissues have been reported to stimulate plant growth (Laslo et al, 2012; Sessitsch et al, 2012; Young et al, 2013). The screening of mushroom growth‐promoting bacteria (MGPB) for mushroom culturing is still limited. Zarenejad et al (2012) screened the casing layer of 14 edible mushroom farms and identified 23 strains that were potent MGPB. Further fieldwork indicated that Pseudomonas putida was the best suited growth‐promoting inoculant for increasing Agaricus bisporus mushroom yield in production farms.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
