Abstract
This study focused on one pathogen, Cryptosporidium parvum, and its occurrence in wastewater. In order to conduct an occurrence study, it was firstly necessary to develop methods for recovery of Cryptosporidium oocysts from wastewater matrices. Due to the differences in matrix composition from raw sewage to tertiary effluents, different methods for recovery and enumeration of oocysts were developed based on matrix quality.A single method was developed for raw sewage and primary influents; a second method for secondary and tertiary effluents; and a third method for biosolids. These methods were used in a survey of Cryptosporidium occurrence at 10 wastewater plants in the U.S. over a 15-month period. To determine if oocysts found in wastewater samples represented a public health risk, cell culture methods were employed to examine infectivity of recovered oocysts.This title belongs to WERF Research Report SeriesISBN: 9781843397182 (Print)ISBN: 9781780404417 (eBook)
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