Abstract
Eight microsatellite loci, recently developed in the species Anisakis pegreffii, were successfully amplified in Anisakis berlandi, sibling species of the A. simplex (s. l.) complex. They were validated on adult specimens (n = 46) of the parasite species, collected from two individuals of the definitive host, the long-finned pilot whale Globicephala melas from New Zealand waters. Among the eight loci scored, one, Anisl 07132, had null alleles in A. berlandi and was thus excluded from the subsequent genetic analysis. Two loci, Anisl 00314 and Anisl 10535, were monomorphic. In addition, as also previously detected in the other species of the A. simplex (s. l.) complex, the Anisl 7 locus was seen to be sex-linked, showing hemizygosity in male specimens. Differential allele frequency distributions of A. berlandi, with respect to those previously observed in A. pegreffii and A. simplex (s. s.), were found at some microsatellite loci. The Anisl 7 locus provided 100% diagnosis between A. berlandi and A. pegreffii, while others resulted in 99% diagnosis between A. berlandi and the other two species. Simple sequence repeat (SSR) loci also allowed us to estimate the genetic differentiation of A. berlandi from A. pegreffii (F st ≈ 0.45, Dc = 0.82) and A. simplex (s. s.) (F st ≈ 0.57, Dc = 0.73). The results suggest that SSRs provide a set of candidate markers for population genetics analysis of A. berlandi, as well as for the investigation, through a multi-locus genotyping approach, of possible patterns of hybridisation/introgression events between A. berlandi and the other two Anisakis species in sympatric conditions.
Highlights
Anisakis berlandi Mattiucci, Cipriani, Webb, Paoletti, Marcer, Bellisario, Gibson, Nascetti, 2014 [10] is a nematode belonging to the A. simplex (s. l.) complex
Allozyme analysis of Anisakis (N = 46 specimens) from the long-finned pilot whale corresponded to A. berlandi, according to alleles found at the diagnostic loci with respect to the other members of the A. simplex (s. l.) complex, i.e. Pep C-192, and Mdh-180,90 [14]
The sequences obtained at the mtDNA cox2 gene (629 bp) of these specimens of A. berlandi matched the sequences deposited in GenBank for A. berlandi from our previous analysis [10]
Summary
Anisakis berlandi Mattiucci, Cipriani, Webb, Paoletti, Marcer, Bellisario, Gibson, Nascetti, 2014 [10] is a nematode belonging to the A. simplex (s. l.) complex. Anisakis berlandi Mattiucci, Cipriani, Webb, Paoletti, Marcer, Bellisario, Gibson, Nascetti, 2014 [10] is a nematode belonging to the A. simplex It was first described as A. simplex C (see [12]), co-infecting with A. simplex Key morphological diagnostic traits between A. berlandi and the other species of the A. simplex complex A procrustes analysis, combining both morphological and genetic datasets on specimens belonging to the three sibling species, showed their clustering into three well-defined groups, corresponding to the three taxa [10]. A concatenated phylogenetic inference, combining both mitochondrial and nuclear sequences datasets, showed the existence of the three species of the A. simplex A concatenated phylogenetic inference, combining both mitochondrial and nuclear sequences datasets, showed the existence of the three species of the A. simplex (s. l.) complex, as distinct phylogenetic lineages [9, 10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
