Abstract
A flashy protein: The flash-induced transient protonation of pyranine has been studied in the presence of the cross-linked double mutant E166C/A228C (red trace), a reduced mutant (blue trace), and wild-type bacteriorhodopsin (gray traces). If helices F and G are cross-linked, there is a delay in both proton release (extracellular side) and proton uptake (cytoplasmic side). Together with flash photolysis and FTIR studies, these data support a rigid-body mechanism of bacteriorhodopsin proton transport.
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