Abstract
Summary The feeder-cell technique, used for tobacco protoplasts, was applied to orange protoplast culture. Cloning efficiency of low-density orange protoplasts seeded above the feeder layer was of the same order of magnitude as that of protoplasts plated at high densities without feeder. The cross-feeding effects between tobacco and orange protoplasts were tested. Both X-irradiated tobacco protoplasts seeded in Nagata & Takebe medium, and non-irradiated tobacco protoplasts seeded in Murashige and Tucker basal medium (without hormones) could support colony formation by the low-density orange protoplasts. The reciprocal feeding experiment, i. e., X-irradiated orange and low-density tobacco protoplasts, succeeded only rarely. This was clearly due to the deleterious effect of the hormones present in the tobacco culture medium.
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