Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-related proteins (CRISPR/Cas) are regarded as the next generation of molecular diagnostic systems. Due to its good biocompatibility, unique cutting activity, ease of operation and design, the CRISPR/Cas system plays an important role in biosensing applications, especially the trans-cutting activity of several Cas effectors. In recent years, CRISPR/Cas, as an emerging molecular recognition component and signal transduction module, has been combined with modern analytical techniques for detecting various analytes. This paper briefly introduces the classification and working principle of CRISPR sensor-related enzymes, summarizes the latest research on the detection of various Cas effectors on single (multiple) targets, and analyzes their application examples in practical and portable sensing from the perspective of multiple detection platforms. Finally, the technical bottleneck and application prospects were summarized.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
