Abstract
Synthetic yeast cell factories provide a remarkable solution for the sustainable supply of a range of products, ranging from large-scale industrial chemicals to high-value pharmaceutical compounds. Synthetic biology is a field in which metabolic pathways are intensively studied and engineered. The clustered, regularly interspaced, short, palindromic repeat-associated (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has emerged as the state-of-the-art gene editing technique for synthetic biology. Recently, the use of different CRISPR/Cas9 systems has been extended to the field of yeast engineering for single-nucleotide resolution editing, multiple-gene editing, transcriptional regulation, and genome-scale modifications. Such advancing systems have led to accelerated microbial engineering involving less labor and time and also enhanced the understanding of cellular genetics and physiology. This review provides a brief overview of the latest research progress and the use of CRISPR/Cas9 systems in genetic manipulation, with a focus on the applications of Saccharomyces cerevisiae cell factory engineering.
Highlights
The development of microbial cell factories have drawn increasing attention because they allow the production in a cost-effective, renewable, and sustainable manner (Xu et al, 2020)
This review mainly focused on the latest advances of the CRISPR/CRISPR-associated protein 9 (Cas9) system in the model yeast S. cerevisiae
Flexibility, and high efficiency in knock-in, the CRISPR/Cas9 system enabled the rapid economic development of a high-throughput industrial yeast cell factory that usually required a lot of genomic integration manipulations
Summary
The development of microbial cell factories have drawn increasing attention because they allow the production in a cost-effective, renewable, and sustainable manner (Xu et al, 2020). Flexibility, and high efficiency in knock-in, the CRISPR/Cas9 system enabled the rapid economic development of a high-throughput industrial yeast cell factory that usually required a lot of genomic integration manipulations. A novel single-nucleotide resolution editing tool was reported (named as CHAnGE) by combining HDR and the CRISPR/Cas9 system that enabled the rapid engineering of S. cerevisiae for improved tolerance to growth inhibitors (Bao et al, 2018).
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