Molecular therapy oncolytics | VOL. 25
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CRISPR-Cas knockout of miR21 reduces glioma growth.

Publication Date Jun 16, 2022

Abstract

Non-coding RNAs, including microRNAs (miRNAs), support the progression of glioma. miR-21 is a small, non-coding transcript involved in regulating gene expression in multiple cellular pathways, including the regulation of proliferation. High expression of miR-21 has been shown to be a major driver of glioma growth. Manipulating the expression of miRNAs is a novel strategy in the development of therapeutics in cancer. In this study we aimed to target miR-21. Using CRISPR genome-editing technology, we disrupted the miR-21 coding sequences in glioma cells. Depletion of this miRNA resulted in the upregulation of many downstream miR-21 target mRNAs involved in proliferation. Phenotypically, CRISPR-edited glioma cells showed reduced migration, invasion, and proliferation in vitro. In immunocompetent mouse models, miR-21 knockout tumors showed reduced growth resulting in an increased overall survival. In summary, we show that by knocking out a key miRNA in glioma, these cells have decreased proliferation capacity both invitro and invivo. Overall, we identified miR-21 as a potential target for CRISPR-based therapeutics in glioma.

Concepts
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CRISPR Genome-editing Technology
High Expression Of miR-21
miRNA In Glioma
Immunocompetent Mouse Models
Multiple Cellular Pathways
Key miRNA
Therapeutics In Cancer
Development Of Therapeutics
Glioma Cells
Regulating Gene Expression

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